ABSTRACT
Objective: To study the chemical constituents from the roots of Clematis manshurica. Methods: Ten compounds were separated and purified by silica gel, Sephadex LH-20, and preparative HPLC. The structures were identified by physicochemical properties and spectral analyses. Results: A total of ten oleanane-type triterpenoid saponins were isolated and identified as clematomandshurica saponin L (1), clematichinenoside A (2), clematochinenoside F (3), clematernoside A (4), clematichinenoside B (5), clematichinenoside C (6), clematomandshurica saponin B (7), clematomandshurica saponin D (8), clematomandshurica saponin C (9), and huzhangoside B (10), respectively. Conclusion: Compound 1 is a new oleanane-type triterpenoid glycoside, and compounds 2-6 are isolated from this plant for the first time.
ABSTRACT
Objective: To investigate the chemical constituents from the ethanol extract of aerial parts of Clematis manshurica. Methods: The compounds were isolated and purified by chromatography on silica gel, ODS, and preparative HPLC. Their structures were elucidated on the basis of chemical and spectroscopic methods, including MS, 1D, and 2D NMR spectral techniques. Results: Seventeen compounds were isolated from the ethanol extract of the aerial parts of C. manshurica, and were identified as (+)-epipinoresinol (1), (+)-pinoresinol (2), (-)-episyringaresinol (3), (+)-medioresinol (4), (+)-pinoresinol-4-O-β-D-glucopyranoside (5), (+)-syringaresinol-4-O-β-D-glucopyranoside (6), (+)-episyringaresinol-4-O-β-D-glucopyranoside (7), matairesinol (8), (+)-lyoniresinol (9), (+)-isolariciresinol (10), (7R, 8R)-4, 7, 9, 9'-tetrahydroxy-3, 3'-diethoxy-8-O-4'-neolignan (11), (7R, 8S)-4, 7, 9, 9'-tetrahydroxy-3, 3'-diethoxy-8-O-4'-neolignan (12), (7S, 8R)-dihydrodehydroconiferyl alcohol (13), luteolin (14), 3″-O-(2‴- methylbutyryl) isoswertisin (15), 2″-O-(2‴-methylbutyryl) isoswertisin (16), and 6″-O-(2‴-methylbutyryl) isoswertisin (17). Conclusion: All compounds are obtained from the aerial parts of this plant for the first time, and compounds 1, 3, 4, 7-9, 11, 12, and 15-17 are firstly isolated from the plants of Clematis L.
ABSTRACT
OBJECTIVE: To establish a rapid and accurate identification method to distinguish species of Clematis. METHODS: First, the registered ITS sequences of Clematis were seeked in the genbank database and compared with those of three species of Clematis' to confirm the stable specific identification site. Second, specific primers were designed, and PCR-SSCP was used to test Clematis. RESULTS: The fingerprints of the samples existed significant differences, indicating that our method was able to tell apart the different species of Clematis. CONCLUSION: PCR-SSCP has the advantages of high specificity and good reproducibility, therefore, it lays the foundation for the further development of accurate identification of Clematis.
ABSTRACT
Objective To study the chemical constituents of Clematis manshurica. MethodsThe extract from the roots and rhizomes of C. manshurica was subjected to chromatography on silica gel and Sephadex LH-20 column. The compounds obtained were identified on the basis of their physicochemical and spectroscopic evidences. ResultsEleven compounds were isolated and their structures were elucidated as squalene (Ⅰ), friedelin (Ⅱ), hexacosoic acid (Ⅲ), ?-sitosterol (Ⅳ), stigmasterol (Ⅴ), oleanolic acid (Ⅵ), ?-daucosterol (Ⅶ), 5-hydroxymethyl-2-furaldehyde (Ⅷ), methyl 3, 4-dihydroxy-phenyl lactate (Ⅸ), 5R-dihydro-5-hydroxymethyl-2(3H)-furanone (Ⅹ), 5R-5-hydroxymethyl-2(5H)-furanone (Ⅺ). ConclusionAll the compounds except for ?-sitosterol are isolated from the plant for the first time.